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各种荧光染料的吸收波长和激发波长

2012/7/11 5:16:32      点击：
荧光分子
颜色
激发波长
发射波长
Alexa Fluor 350
Blue
346 nm
442 nm
Alexa Fluor 405
Blue
402 nm
421 nm
Pacific Blue
Blue
410 nm
455 nm
DAPI
Blue
340 nm
488 nm
AMCA
Blue
350 nm
450 nm
Cascade Blue
Blue
400 nm
420 nm
EviTag(TM) quantum dots - Lake Placid Blue (490 nm)
Blue
<400 nm
490 nm
Fluoroblue
Blue
350 nm
450 nm
Image-Blue
Blue
380 nm
465 nm
Marina Blue
Blue
365 nm
460 nm
Pacific Blue
Blue
410 nm
455 nm
Cy2
Green
491 nm
509 nm
Alexa Fluor 430
Green
434 nm
539 nm
Alexa Fluor 488
Green
495 nm
519 nm
Alexa Fluor 488 FluoroNanogold
Green
495 nm
519 nm
Alexa Fluor 500
Green
503 nm
525 nm
Alexa Fluor 514
Green
518 nm
540 nm
FITC
Green
490 nm
520 nm
Fluorescein
Green
494 nm
518 nm
HNP
Green
290 nm
509 nm
5-FAM
Green
494 nm
522 nm
6-FAM
Green
492 nm
517 nm
BODIPY FL
Green
505 nm
513 nm
CMNB-caged Fluorescein
Green
494 nm
518 nm
Cyanine 2
Green
492 nm
510 nm
DTAF
Green
495 nm
520 nm
DTAF/FITC
Green
495 nm
520 nm
dUTP-Biotin and Avidin FITC
Green
495 nm
520 nm
dUTP-FITC
Green
495 nm
520 nm
EviTag(TM) quantum dots - Adirondack Green (520 nm)
Green
<400 nm
520 nm
EviTag(TM) quantum dots - Catskill Green (540 nm)
Green
<400 nm
540 nm
FITC (Fluorescein)
Green
495 nm
520 nm
Fluorescein-5-EX, succinimidyl ester (FSE)
Green
495 nm
520 nm
Hi FITC
Green
495 nm
520 nm
JOE
Green
520 nm
548 nm
MFP488
Green
501 nm
523 nm
Oregon Green 488
Green
496 nm
524 nm
Oregon Green 514
Green
511 nm
530 nm
Qdot 525
Green
488 nm
515 nm
TET
Green
521 nm
536 nm
CAL Fluor(R) Gold 540
Green
522 nm
544 nm
NL493
Green
493 nm
514 nm
GFP
Green
395/475 nm
509 nm
Cy3
Yellow
548 nm
562 nm
Cy3B
Yellow
558 nm
572 nm
Alexa Fluor 532
Yellow
531 nm
554 nm
Alexa Fluor 546
Yellow
556 nm
573 nm
Alexa Fluor 555
Yellow
555 nm
565 nm
R-Phycoerythrin
Yellow
480, 546, 565 nm
578 nm
B-Phycoerythrin
Yellow
546, 565 nm
575 nm
HNP/Fast Red TR
Yellow
290 nm
562 nm
5-TAMRA
Yellow
546 nm
574 nm
Atto 550
Yellow
554 nm
576 nm
CAL Fluor(R) Orange 560
Yellow
538 nm
559 nm
Cyanine 3
Yellow
550 nm
570 nm
DyLight 547
Yellow
547 nm
574 nm
EviTag(TM) quantum dots - Birch Yellow (580 nm)
Yellow
<400 nm
580 nm
EviTag(TM) quantum dots - Hops Yellow (560 nm)
Yellow
<400 nm
560 nm
HEX
Yellow
535 nm
556 nm
NED
Yellow
546 nm
575 nm
Qdot 565
Yellow
<500 nm
565 nm
Quasar(R) 570
Yellow
548 nm
566 nm
R-Phycoerythrin (R-PE)
Yellow
488 nm
575 nm
TRITC (Rhodamine)
Yellow
550 nm
570 nm
VIC
Yellow
538 nm
554 nm
NL557
Yellow
557 nm
574 nm
Phycoerythrin
Yellow
480-566 nm
575 nm
Cy3.5
Orange
581 nm
596 nm
Alexa Fluor 568
Orange
578 nm
603 nm
ROX
Orange
575 nm
602 nm
DyeMer 488/605
Orange
502 nm
609 nm
DyeMer 488/615
Orange
502 nm
615 nm
CAL Fluor(R) Red 590
Orange
569 nm
591 nm
CAL Fluor(R) Red 610
Orange
569 nm
610 nm
Cyanine 3.5
Orange
581 nm
596 nm
DyeMer 488/605
Orange
488 nm
605 nm
DyeMer 488/615
Orange
488 nm
615 nm
EviTag(TM) quantum dots - Fort Orange (600 nm)
Orange
<400 nm
600 nm
Lissamine Rhodamine
Orange
570 nm
590 nm
MFP555
Orange
560 nm
585 nm
Qdot 585
Orange
532 nm
585 nm
Qdot 605
Orange
<600 nm
605 nm
ROX
Orange
575 nm
602 nm
R-Phycoerythrin-Texas Red (PE-Texas Red, ECD)
Orange
480-565 nm
615 nm
Rhodamine Red-X
Orange
570 nm
590 nm
TMR red
Red
540 (520-560) nm
580 (570-620) nm
Cy5
Red
646 nm
664 nm
Cy5.5
Red
673 nm
692 nm
CypHer5E
Red
644 nm
663 nm
Alexa Fluor 594
Red
590 nm
617 nm
Alexa Fluor 594 FluoroNanogold
Red
590 nm
617 nm
Alexa Fluor 610
Red
612 nm
628 nm
Alexa Fluor 610–R-PE
Red
496, 546, 565 nm
630 nm
Alexa Fluor 633
Red
632 nm
647 nm
Alexa Fluor 635
Red
633 nm
647 nm
Alexa Fluor 647
Red
650 nm
668 nm
Alexa Fluor 647–R-phycoerythrin
Red
496, 546, 565 nm
668 nm
Alexa Fluor 660
Red
663 nm
690 nm
Texas Red
Red
596 nm
620 nm
DyeMer 488/630
Red
502 nm
630 nm
Allophycocyanin
Red
650 nm
660 nm
PI
Red
535 nm
617 nm
Allophycocyanin (APC)
Red
650 nm
660 nm
Allophycocyanin-Cyanine 5
Red
633 nm
670 nm
Allophycocyanin-Cyanine 5.5
Red
633 nm
690 nm
Atto 647
Red
645 nm
669 nm
CAL Fluor(R) Red 635
Red
618 nm
637 nm
Cyanine 5
Red
650 nm
670 nm
DyLight 647
Red
647 nm
673 nm
DyeMer 488/630
Red
488 nm
630 nm
Dyomics 647
Red
660 nm
673 nm
Lightcycler Red- 640
Red
625 nm
640 nm
PBXL-1
Red
545 nm
666 nm
PBXL-3
Red
614 nm
662 nm
PerCP (Peridinin Chlorophyll)
Red
490 nm
675 nm
PerCP-Cy5.5
Red
488 nm
695 nm
Qdot 655
Red
<620 nm
655 nm
Quantum Red
Red
488 nm
660 nm
Quasar(R) 670
Red
647 nm
667 nm
R-Phycocyanin (R-PC)
Red
614 nm
647 nm
R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red)
Red
488 nm
660 nm
Sulforhodamine
Red
585 nm
620 nm
Texas Red
Red
596 nm
620 nm
Cyanine Q (Cyanine 5.18)
Red
488 nm
670 nm
NL637
Red
637 nm
658 nm
Allophycocyanin
Red
620-650 nm
660-670 nm
CytoCy5S
Red
628 nm
638 nm
Alexa Fluor 680
Far-Red
679 nm
702 nm
Alexa Fluor 680–Allophycocyanin
Far-Red
650 nm
702 nm
Alexa Fluor 680-R-phycoerythrin
Far-Red
555 nm
700 nm
Alexa Fluor 700
Far-Red
702 nm
723 nm
Alexa Fluor 700–Allophycocyanin
Far-Red
650 nm
723 nm
Alexa Fluor 750
Far-Red
749 nm
775 nm
Alexa Fluor 750–Allophycocyanin
Far-Red
650 nm
775 nm
Allophycocyanin-Cyanine 7
Far-Red
633 nm
760 nm
Cyanine 5.5
Far-Red
678 nm
703 nm
Cyanine 7
Far-Red
710 nm
805 nm
IRDYE700DX
Far-Red
689 nm
700 nm
IRDYE800
Far-Red
778 nm
795 nm
IRDYE800CW
Far-Red
774 nm
800 nm
NIRD700
Far-Red
682 nm
701 nm
Qdot 705
Far-Red
<700 nm
705 nm
Qdot 800
Far-Red
<600 nm
800 nm
R-Phycoerythrin-Cyanine 5.5
Far-Red
488 nm
703 nm
R-Phycoerythrin-Cyanine 7 (PC7)
Far-Red
488 nm

805 nm